BL-2

Cat.No.: CSC-C0618

Species: Human

Source: bone marrow

Morphology: small, round to polymorphic cells, growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0618

Description

Established in 1979 from the bone marrow of a 7-year-old Caucasian boy with non endemic Burkitt lymphoma (stage III) after chemo- and radiotherapy; cells are described to express no Epstein-Barr virus (EBV) antigen and that somatic immunoglobulin gene hypermutation can be induced

Species

Human

Source

bone marrow

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

small, round to polymorphic cells, growing in suspension

Karyotype

Human flat-moded near-diploid karyotype with 3% polyploidy; 44-47<2n>XY, der(1)t(1;7)(q32;q11.2), der(6)t(1;6)(q21;q25), t(8;22)(q24;q11.2), del(11)(q24.2); carries t(8;22) effecting juxtaposition of MYC with IGL@ (IGH); matches published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa -, sm/cylambda +
Viruses: PCR: EBV -, HBV -, HC

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 12 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the difference between cytogenetics and karyotype?

Cytogenetics is a field of study that deals with chromosomes and related abnormalities. Chromosome analysis is also known as karyotyping and involves the pairing of homologous chromosomes.

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