BF-F3

Cat.No.: CSC-C6158X

Species: Mouse

Source: hybridoma (anti-rat fiber/myosin)

Morphology: single round cells in suspension and partially adherent, but trypsin/EDTA is not necessary to detach the cells

Culture Properties: suspension

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Cat.No.

CSC-C6158X

Description

This hybridoma cell line was described as secreting a mouse monoclonal antibody (IgM) reacting with adult rat type 2B fibers in immunofluorescence and with rat type 2B MHCs (myosin heavy chain) in Western blotting; established in 1983 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles

Species

Mouse

Source

hybridoma (anti-rat fiber/myosin)

Recommended Medium

90% DMEM + 10% h.i.FBS

Culture Properties

suspension

Morphology

single round cells in suspension and partially adherent, but trypsin/EDTA is not necessary to detach the cells

Karyotype

Murine chromosome morphology

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of a IgM monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 6 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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