BETA-TC-3

Cat.No.: CSC-C1092

Species: Mouse

Source: pancreas insulinoma (beta-tumor)

Morphology: adherent cells growing as multilayers

Culture Properties: multilayer

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Cat.No.

CSC-C1092

Description

Established from pancreatic insulinoma excised from a transgenic mouse carrying hybrid insulin-SV40 large T genes in 1988; described as producing pro-insulin I and II processed into mature insulin (inducible 30-fold by glucose); however, the Creative Bioarray did not verify the production of insulin

Species

Mouse

Source

pancreas insulinoma (beta-tumor)

Recommended Medium

82.5% DMEM + 15% horse serum. + 2.5% h.i.FBS

Culture Properties

multilayer

Morphology

adherent cells growing as multilayers

Karyotype

Murine hypotetraploid karyotype - 74(70-78)<4n> - 2-4 centric fusion markers and 1-3 dmin present

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 2.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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