BAG-12G2

Cat.No.: CSC-C6159X

Species: Mouse

Source: hybridoma (CDw78)

Morphology: single round cells growing in suspension (some cells adhere partly)

Culture Properties: suspension

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Cat.No.

CSC-C6159X

Description

Described as secreting a mouse monoclonal antibody (IgG2akappa) CDw78; the cell line was established in 1988 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with human peripheral blood lymphocytes from a patient with high numbers of gamma-delta T cells

Species

Mouse

Source

hybridoma (CDw78)

Recommended Medium

90% DMEM + 10% h.i.FBS + HFCS ("Hybridoma Fusion and Cloning Supplement")

Culture Properties

suspension

Morphology

single round cells growing in suspension (some cells adhere partly)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of a IgG2a monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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