AP-1060

Cat.No.: CSC-C0596

Species: Human

Source: acute myeloid leukemia

Morphology: single round cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0596

Description

Established from the bone marrow of a 45-year-old man with acute promyelocytic leukemia (APL = AML FAB M3) in fourth relapse in 1998; cells were immortalized using ethyl-nitrosourea; cells are described to be constitutively dependent on GM-CSF and IL-3 or G-CSF; cells carry and express the t(15;17)(q22;q11.2) PML-RARA fusion gene

Species

Human

Source

acute myeloid leukemia

Recommended Medium

70% SuperCult® IMDM + 20% h.i. FBS + 10% vol conditioned medium of cell line 5637

Culture Properties

suspension

Morphology

single round cells growing in suspension

Karyotype

Human near-diploid karyotype with 3% polyploidy - 46(42-47)<2n>XY, t(3;14)(p21;q11.2), add(5)(q33), del(6)(q25), add(12)(p13), t(15;17)(q22;q21); carries t(15;17) with rearrangement of PML and RARA; resembles published karyotype

Quality Control

Mycoplasma: negative in microbiological culture, PCR assays
Immunology: CD3 -, CD13 +, CD14 -, CD19 -, CD33 +, HLA-DR -
Viruses: PCR: EBV -, HBV -, HCV -, HIV -, HTLV-I/II -, SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the difference between clonal and subclonal?

Clonal mutations are shared by all cancer cells, whereas subclonal mutations are present only in a subset.

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