A4-1077

Cat.No.: CSC-C6164X

Species: Mouse

Source: hybridoma (anti-fiber/myosin)

Morphology: single round cells, lightly adherent

Culture Properties: suspension

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Cat.No.

CSC-C6164X

Description

Described as secreting a mouse monoclonal antibody (IgG1) reacting with human nascent secondary fibers and adult fast fibers and myosin heavy chains in Western blots (does not detect rat); the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin

Species

Mouse

Source

hybridoma (anti-fiber/myosin)

Recommended Medium

85% DMEM + 15% h.i.FBS + 1x non-essential amino acids

Culture Properties

suspension

Morphology

single round cells, lightly adherent

Karyotype

Murine flat-moded hypertetraploid/pentaploid karyotype with 10% polyploidy - 90(81-96)<4n> - 2-3 centric fusion markers and 1-2 dmin present in most cells

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase positive; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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