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Cat.No.: CSC-C6187X

Species: Mouse

Source: hybridoma

Morphology: spheroidal cells growing singly in suspension, or occasionally loosely adherent

Culture Properties: suspension

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Cat.No.

CSC-C6187X

Description

Cell line was established by fusion of mouse NS-1 cells with spleen from a BALB/c mouse immunized with enriched retinal cell membranes; described as secreting a mouse monoclonal antibody (IgM) reacting with the chicken dorsal retina cytoplasmic membranes (E12) and binding outer photoreceptor elements of adult vertebrate retina (inclusive chick, mouse, rat)

Species

Mouse

Source

hybridoma

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS + HFCS ("Hybridoma Fusion and Cloning Supplement") for the first 1-2 weeks post-thawing

Culture Properties

suspension

Morphology

spheroidal cells growing singly in suspension, or occasionally loosely adherent

Karyotype

Murine flat-moded hypertetraploid karyotype - 94-102<4n> - no centric fusion markers present

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: secretion of a IgM monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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