TI-1

Cat.No.: CSC-C6168X

Species: Mouse

Source: hybridoma (anti-rat fiber/troponin I)

Morphology: single round cells in suspension; culture contains almost always some cell debris and dead cells

Culture Properties: suspension

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C6168X

Description

Described as secreting a mouse monoclonal antibody (IgG2b) reacting with rat adult cardiac fibers in immunofluorescence and with rat adult cardiac troponin I in Western blotting; this cell line was produced by fusing NSO myeloma cells with spleen cells from a BALB/c mouse immunized with myofibrillar preparation from hypothyroid rabbit ventricle

Species

Mouse

Source

hybridoma (anti-rat fiber/troponin I)

Recommended Medium

90% DMEM + 10% horse serum.

Culture Properties

suspension

Morphology

single round cells in suspension; culture contains almost always some cell debris and dead cells

Karyotype

Murine hypertetraploid/hypopentaploid karyotype - 92(87-92), 1-3 dm present in most cells

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG2b monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% horse serum, 10% DMSO at about 3-4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

Ask a Question