BF-34

Cat.No.: CSC-C6161X

Species: Mouse

Source: hybridoma (anti-rat fiber/myosin)

Morphology: single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells)

Culture Properties: suspension

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Cat.No.

CSC-C6161X

Description

This hybridoma cell line was described as secreting a mouse monoclonal antibody (IgG1) that reacts with rat neonatal fibers and, to a lower extent, with all rat adult type 2 fibers in immunofluorescence; it reacts specifically with neonatal MHCs (myosin heavy chain) in Western blotting; established in 1984 by fusion of NSO myeloma cells with spleen cells from a BALB/c mouse immunized with purified myosin from fetal bovine muscles

Species

Mouse

Source

hybridoma (anti-rat fiber/myosin)

Recommended Medium

90% DMEM + 10% horse serum. + 2 mM L-glutamine

Culture Properties

suspension

Morphology

single, round cells in suspension (some cells adhere partly, but trypsin is not necessary to detach these cells)

Karyotype

Murine chromosome morphology

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG1 monoclonal antibody
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV -

Storage and Shipping

Frozen with 70% medium, 20% horse serum, 10% DMSO at about 1.5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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