A4-1025

Cat.No.: CSC-C6173X

Species: Mouse

Source: hybridoma (anti-fiber/myosin)

Morphology: round single cells in suspension, partly in clusters, lightly adherent

Culture Properties: suspension

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Cat.No.

CSC-C6173X

Description

Described as secreting a mouse monoclonal antibody (IgG2a) detecting all fibers in skeletal muscle and myosin heavy chains on Western blots and detecting mammalian, chicken, zebrafish, axolotl, xenopus skeletal muscle myosin heavy chains; the cell line was established in 1985 by fusion of X63AG8.653 cells with spleen cells from a BALB/c mouse that had been immunized with human skeletal muscle myosin

Species

Mouse

Source

hybridoma (anti-fiber/myosin)

Recommended Medium

90% DMEM + 10% h.i.FBS + 1x non-essential amino acids

Culture Properties

suspension

Morphology

round single cells in suspension, partly in clusters, lightly adherent

Karyotype

Murine hypertetraploid karyotype with 5% polyploidy - 84(79-84) - one large submetacentric and up to 5 centric fusion markers present

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization assays
Immunology: secretion of a IgG2a monoclonal antibody
Viruses: ELISA: reverse transcriptase positive

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 3 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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