ActoFactor™ Recombinant Mouse Growth hormone

CSC-CTK0781

GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. Mouse recombinant Growth Hormone produced in E. coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids and having a molecular mass of 22 kDa.

Mouse

Mouse Gh expressed in E.coli

22 kDa

CAT# CSC-CTK0781-100 (100 μg); CAT# CSC-CTK0781-500 (500 μg)

E.coli

Greater than 95% as determined by SDS-PAGE and RP-HPLC analysis.

Recombinant mouse Growth Hormone is fully biologically active when compared to a standard Human Growth Hormone (3 units/mg).

N/A

Sterile-filtered, white, lyophilized (freeze-dried) powder. Lyophilized from a protein solution in 50 mM Tris-HCl, 150 mM NaCl buffer (pH 8).

Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.

The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze-thaw cycles.

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