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ActoFactor™ Recombinant Human Interleukin 1, alpha
- Specification
Cat.No.
CSC-CTK0070
Description
Interleukin 1 is a name that designates two proteins, IL-1a and IL-1b, that are the products of distinct genes but recognize the same cell surface receptors. The proteins are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17 kDa. IL-1a and IL-1b are potent pro-inflammatory cytokines that induce a wide variety of biological activities on different cell types. Recombinant human IL-1a contains 159 amino acids and predicted molecular mass of 18 kDa.
Species
Human
Product Overview
Human IL1A expressed in E.coli
Sequence
A DNA sequence encoding human IL-1a was expressed in E. Coli.
Size
CAT# CSC-CTK0070-2 (2 μg); CAT# CSC-CTK0070-10 (10 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The ED50 as measured in a cell proliferation assay using the murine helper T cell line, D10.G4.1, is typically 3 - 7 pg/mL.
Endotoxin Level
Endotoxin level is less than 0.1 ng per mg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a sterile filtered solution in 20 mM Tris, pH 8.3, containing 50 mg of bovine serum albumin per 1 mg of cytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin is added to the vial to prepare a stock solution of no less than 1 mg/mL of the cytokine.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted IL-1b can be stored under sterile conditions at 2o to 4 °C for one month or at -20°to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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