Featured Products
- HCC-78
- HDLM-2
- DOHH-2
- L-540
- MX-1
- NALM-6
- NB-4
- CAL-51
- SNB-19
- KYSE-520
- MKN-45
- BA/F3
- MS-5
- HCEC-B4G12
- NK-92
- PA-TU-8988S
- MONO-MAC-1
- PA-TU-8902
- Human Microglia
- Human Hepatic Stellate Cells
- Human Skeletal Muscle Cells (DMD)
- Human Schwann Cells
- Human Oral Keratinocytes (HOK)
- Human Cardiomyocytes
- Human Small Intestinal Epithelial Cells
- Human Colonic Epithelial Cells
- Human Intestinal Fibroblasts
- Primary Human Large Intestine Microvascular Endothelial Cells
- Human Small Intestinal Microvascular Endothelial Cells
- Human Retinal Pigment Epithelial Cells
- Human Hepatocytes
- Cynomolgus Monkey Lung Microvascular Endothelial Cells
- Cynomolgus Monkey Vein Endothelial Cells
- C57BL/6 Mouse Primary Mammary Epithelial Cells
- C57BL/6 Mouse Vein Endothelial Cells
- Rat Primary Kidney Epithelial Cells
- Rat Gingival Epithelial Cells
- Rabbit Lung Endothelial Cells
Our Promise to You
Guaranteed product quality, expert customer support
ActoFactor™ Recombinant Human Fibroblast growth factor 7 (plant)
- Specification
Cat.No.
CSC-CTK0364
Description
Keratinocyte Growth Factor (KGF, FGF-7, HBGF-7) is a member of the FGF family of 23 related mitogenic proteins. These proteins play a central role during embryonic development and postnatal growth and tissue repair, by promoting cellular proliferation and differentiation. KGF specifically stimulates epithelial cells and keratinocytes, it is the most potent growth factor identified thus far for skin keratinocytes. KGF takes part in the development of kidney and lung, angiogenesis and has a key role in wound healing following skin injuries. It shows considerable species cross-reactivity (mouse, monkey, porcine cells) as the other members of the FGF family. Recombinant human KGF produced in plant contains 164 amino acids and a 16 a.a. histidine- based tag for a total length of 180 a.a., and has a predicted molecular mass of 21.2 kDa including the His-tag. As a result of glycosylation, the recombinant protein migrates as two bands with an apparent molecular mass of 30 and 38 kDa in SDS-PAGE.
Species
Human
Product Overview
Human FGF7 expressed in Endosperm tissue of barley grain (Hordeum vulgare)
Molecular Mass
21.2 kDa
Size
CAT# CSC-CTK0364-10 (10 μg); CAT# CSC-CTK0364-50 (50 μg)
Expression System
Endosperm tissue of barley grain (Hordeum vulgare)
Purity
Greater than 95% as determined by SDS-PAGE analysis.
Activity
Each batch of KGF is tested for bioactivity and verified to have comparable activity to a commercial source. The bioactivity of recombinant human KGF was determined by its dose dependent effect on proliferation of 4MBr5 rhesus monkey epithelial cells. The ED50 for this effect was found to be 2.5 x 10^5 units/mg. Optimal concentration should be determined for specific applications and cell lines.
Endotoxin Level
Less than 0.05 EU/μg.
Formulation
Sterile filtered through a 0.2 μm filter. Lyophilized from PBS (pH 7.2).
Reconstitution
Please centrifuge the vial before opening. It is recommended to reconstitute the lyophilized protein in sterile water to a concentration of no less than 100 μg/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
- Q & A
- Customer Review
Ask a Question
Write your own review
ONLINE INQUIRY
