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ActoFactor™ Recombinant Human Fibroblast growth factor 7 (E. coli)
- Specification
Cat.No.
CSC-CTK0363
Description
KGF is a potent specific mitogen for many epithelial cells but not for fibroblasts and endothelial cells. KGF is thought to play an important role in the paracrine growth control of normal epithelial cells. KGF stimulates the proliferation of primary and secondary human keratinocytes to the same extent as EGF. Recombinant human KGF-1 (FGF-7) produced in E. coli is a single, non- glycosylated polypeptide chain of 164 amino acids and a molecular mass of 18,995 Dalton.
Species
Human
Product Overview
Human FGF7 expressed in E.coli
Molecular Mass
19 kDa
Size
CAT# CSC-CTK0363-10 (10 μg); CAT# CSC-CTK0363-50 (50 μg)
Expression System
E.coli
Purity
Greater than 96% as determined by SDS-PAGE and RP-HPLC analysis.
Activity
Recombinant human Keratinocye Growth Factor is fully biologically active when compared to standards. The ED50, calculated by the dose-dependant stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake), is 1 x 10^5 units/mg.
Endotoxin Level
Less than 1 EU/μg.
Formulation
White (freeze-dried) powder. Lyophilized from a sterile-filtered protein solution in 20 mM phosphate buffer (pH 8.0), 1 M NaCl.
Reconstitution
Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use.
Storage & Stability
The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu KGF should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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