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ActoFactor™ Recombinant Human Interleukin 4
- Specification
Cat.No.
CSC-CTK0090
Description
Interleukin-4 (IL-4) is a pleiotrophic cytokine produced by activated T cells, mast cells and basophils. IL-4 stimulates the activation and differentiation of B cells as well as other hematopoietic and non-hematopoietic cells. Recombinant human IL-4 is a 14 kDa protein containing 130 amino acid residues.
Species
Human
Product Overview
Human IL4 expressed in E.coli
Sequence
A DNA sequence encoding mature human IL-4 was expressed in E. coli.
Size
CAT# CSC-CTK0090-10 (10 μg); CAT# CSC-CTK0090-50 (50 μg)
Expression System
E.coli
Purity
Greater than 97% as determined by SDS-PAGE and visualized by silver stain
Activity
The biological activity of human IL-4 was measured in a cell proliferation assay using the factor-dependent human cell line, TF-1. The ED50 for this effect is typically 0.05 - 0.2 ng/mL.
Endotoxin Level
Endotoxin level is less than 0.1 ng per μg of the cytokine, as determined by the LAL method.
Formulation
Lyophilized from a sterile-filtered solution in PBS containing 50 μg of bovine serum albumin per 1 μg of cytokine.
Reconstitution
It is recommended that sterile phosphate-buffered saline containing at least 0.1% human serum albumin or bovine serum albumin be added to the vial to prepare a stock solution of no less than 5 μg /mL of the cytokine.
Storage & Stability
The lyophilized powder is stable for greater than six months at -20°C to -70°C. Reconstituted IL-4 can be stored under sterile conditions at 2°C to 4°C for one month or at -20°C to -70°C for three months without detectable loss of activity. Avoid repeated freezing and thawing.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.
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