Diabetic Mouse Alveolar Epithelial Cells

Diabetic Mouse Alveolar Epithelial Cells

Cat.No.:CSC-C9320J

Source: Lung

Cell Type: Epithelial

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Cat.No.
CSC-C9320J
Description
Diabetic Mouse Alveolar Epithelial Cells from Creative Bioarray are isolated from the lung of Diabetic (db/db) mice. Diabetic Mouse Alveolar Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in Creative Bioarray’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x10^6cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.
Source
Lung
Cell Type
Epithelial
Disease
Diseased
Storage
Liquid Nitrogen (-180 °C).
Quality Control
Diabetic Mouse Alveolar Epithelial Cells are characterized by immunofluorescent staining with antibodies of E-cadherin, ZO-1 (Catalog 617300, Life Technology) or Supernatant (T1 alpha). T1 alpha was identified by a monoclonal antibody previously shown to be specific for an antigen expressed by alveolar epithelial type I cells. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be expanded for 3-7 passages at a split ratio of 1:2 under the cell culture conditions specified by Creative Bioarray. Repeated freezing and thawing of cells is not recommended.
Storage and Shipping
Creative Bioarray ships frozen cells on dry ice. On receipt, immediately transfer frozen cells to liquid nitrogen (-180 °C) until ready for experimental use. Never can cells be kept at -20 °C.
Citation Guidance
If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

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