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Cat.No.: CSC-C0445

Species: Human

Source: B cell leukemia

Morphology: single, small, round cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0445

Description

Established from the peripheral blood of an 11-year-old boy with acute lymphoblastic leukemia (ALL FAB L2) in 1990

Species

Human

Source

B cell leukemia

Recommended Medium

90% RPMI-1640 + 10% h.i. FBS

Culture Properties

suspension

Morphology

single, small, round cells growing in suspension

Karyotype

Human hyperdiploid karyotype with 12% polyploidy - 47/48<2n>X, -Y/XYqh+, +7, +14, dup(1)(q21.1/21.2q23.1/23.2), t(2;4)(q2?2;q2?5), del(6)(q27), t(8;14)(q24;q32), del(13)(q34), add(22)(q13) - extensive subclonal variation - carries t(8;14), +7, and dup(1q)

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 (+), CD38 +, cyCD79a +, CD138 +, HLA-DR (+)
Viruses: ELISA: reverse transcriptase negative; PCR: EBV -, HB

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 4 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What are the disadvantages of ISH?

Some of the disadvantages of ISH are given as follows. Specificity, the specificity of ISH is dependent on the design and quality of the probe used. If the probe is not specific to the target sequence, cross-hybridization with other nucleic acids can occur, leading to false positives.

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Average Rating: 5.0 | 1 Scientist has reviewed this product

Trustworthy

We purchased the tumor cell product to study the role of specific genetic mutations or aberrations in the development and progression of lymphoma. The product has proven to be trustworthy.

20 Feb 2023

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