SK-MM-2

Cat.No.: CSC-C0465

Species: Human

Source: plasma cell leukemia

Morphology: round single cells growing in suspension

Culture Properties: suspension

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Cat.No.

CSC-C0465

Description

Established from the peripheral blood of a 54-year-old man with plasma cell leukemia (Igkappa) (refractory, terminal state) in 1982; plasma cell leukemia is related to multiple myeloma

Species

Human

Source

plasma cell leukemia

Recommended Medium

80% RPMI-1640 + 20% h.i. FBS

Culture Properties

suspension

Morphology

round single cells growing in suspension

Karyotype

Human bimodal hypodiploid/hypotetraploid karyotype with 9% polyploidy (including a subpopulation with 32-64N) - 42(38-43)<2n>XX, -13, -18, -22, del(1)(p32p36), der(2)t(1;2)(p32;q3?1)t(1;13)(p36q1?4), der(4;14)(4pter->4p15::4p11->4p10::14q10->14q22::4q11-1

Quality Control

Mycoplasma: negative in DAPI, microbiological culture, RNA hybridization, PCR assays
Immunology: CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD34 -, CD37 -, CD38 +, CD80 -, HLA-DR -, sm/cyIgG -, sm/cyIgM -, smkappa -, cykappa +, sm/cylambda -
Viruses: ELISA: r

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 5 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What is the difference between IHC and ISH?

IHC will allow you to look into the spatial localization of proteins in tissue while ISH allows you to study different types of RNA localization. We offer both chromogenic or fluorescence (ISH or FISH) modalities for all techniques.

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