CAL-120

Cat.No.: CSC-C0488

Species: Human

Source: breast adenocarcinoma

Morphology: heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers

Culture Properties: monolayer

Datasheet

Inquire for Price

Specification
Q & A
Customer Review

Cat.No.

CSC-C0488

Description

Established from the pleural effusion of a 43-year-old woman with metastatic breast adenocarcinoma in 1991

Species

Human

Source

breast adenocarcinoma

Recommended Medium

90% DMEM+ 10% h.i.FBS

Culture Properties

monolayer

Morphology

heterogenous population with epithelial-like and fibroblast-like adherent cells growing in monolayers

Karyotype

Human hypertetraploid karyotype with 20% polyploidy 98(88-98)<4n>XXX/XXXX, -1, -2, -2, -4, +5, +5, +6, -9, -9, +10, +11, -12, -12, -13, -13, -14, -15, -16, -16, -20, -21, -22, +16-18mar, + 1-2 dmin, add(X)(p2?2), add(1)(q22), del(3)(q22), add(3)(p1?4), de

Quality Control

Mycoplasma: negative in microbiological culture, RNA hybridization, PCR assays
Immunology: cytokeratin +, cytokeratin-7 -, cytokeratin-8 +, cytokeratin-17 -, cytokeratin-18 +, cytokeratin-19 +, desmin -, endothel -, EpCAM +, GFAP -, neurofilament -, vimen

Storage and Shipping

Frozen with 70% medium, 20% FBS, 10% DMSO at about 1 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen

Citation Guidance

If you use this products in your scientific publication, it should be cited in the publication as: Creative Bioarray cat no. If your paper has been published, please click here to submit the PubMed ID of your paper to get a coupon.

What should I do in case of cross-contamination of cells during cell culture?

If cross-contamination is suspected, cytogenetic identification can be done.

Ask a Question

Average Rating: 5.0 | 1 Scientist has reviewed this product

Good product

I have purchased CAL-120 products for scientific research and recommended it to others.

15 Sep 2021

Ease of use
After sales services
Value for money

Write your own review

You May Also Need

CM-1010X

CS-001Y

MCF7-382

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with pBact, expression vector under control of the human beta actin promotor, resistant to G418S.;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6288W INQUIRY

View Product

MCF7-422

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with ER-pBact, human estrogen receptor cDNA, under control of the human beta actin promotor, resistant to G418S.;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6289W INQUIRY

View Product

MCF7-432

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the exon 4 defective variant of ER-pBact cDNA, human estrogen receptor cDNA, under control of the human beta actin promotor resistant to G418S.;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6290W INQUIRY

View Product

MCF7-488X1

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the exon 5 defective variant of human estrogen receptor cDNA, cloned in pZeoSV vector, resistant to zeocin;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6291W INQUIRY

View Product

MCF7-490X1

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the exon 4 defective variant of human estrogen receptor cDNA, cloned in pZeoSV vector, resistant to zeocin;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6292W INQUIRY

View Product

MCF7-492X1

Description: Species: human, Caucasian female 69 years old;
Tissue: breast;
Tumor: adenocarcinoma;
Transfected with the pZeoSV-LacZ vector, resistant to zeocin, control for MCF7-488X1 and MCF7-490X1.;
Original line: MCF7;
Derived from: pleural effusion

Cat#: CSC-6293W INQUIRY

View Product

For research use only. Not for any other purpose.